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KMID : 0900920000240010069
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Korean journal of Animal Reproduction
2000 Volume.24 No. 1 p.69 ~ p.76
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Effects of Embryo Density on Development of In Vitro Produced Bovine Embryos
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Song Sang-Hyun
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Abstract
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This study was carried out to improve of effective culture system on development of IVM/IVF/IVC bovine embryos. The cumulus-oocyte-complexes (COCs) collected from Korean cattle ovaries harvested at a local abattoir were matured in 50 {mu}ell of TCM199 supplemented with 10% fetal bovine serum (FBS) and hormones (35 mutextrm{g}/mell FSH, 10 mutextrm{g}/mell LH, 1mutextrm{g}/mell estradiol 17 beta under paraffin oil at 39$^{circ}C in a humidified atmosphere of 5% CO_2in air. At 24 hrs after culture, matured oocytes were fertilized in vitro for 22~24 hrs with motile semen in which obtained by centrifugation of a frozen thawed semen on Percoll-density gradients (45% vs. 90%) at 500 g for 20 min. The presumptive zygotes were divided into three experimental groups. Single egg (Group 1), 25 (Group 2) or 50 eggs (Group 3) were cultured on cumulus cell in 50 {mu}ell TCM199 supplement with 10% FBS for 6~9 days after fertilization. In vitro developmental rates into the blastocysts in the groups 2 and 3 were significantly (P＜0.05) higher than those of group 1 (37,27 vs. 6%, respectively). Cell number of blastocysts obtained in groups 2 and 3 at day 8 were significantly (P{mu}ell) resulted in higher developmental competence and cell number of bovine blastocysts produced in vitro than those the culture of single embryos with cumulus cells.
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KEYWORD
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Bovine, Embryo density, Media volume, In vitro fertilization, In vitro culture
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